Main Article Content

Abstract

Hepatocellular damage is one of the histopathologic features of malaria infection. The presence of large amounts of hemozoin pigment in the liver is associated with hepatocellular damage. Curcuma xanthorriza Roxb / curcumin as an anti-inflammatory and natural antioxidant which has a hepatoprotector function. The purpose of these study was to determine the effect of curcuma rhizome (Curcuma xanthorrhiza Roxb) extract on hemozoin pigment in the liver tissue of BALB/C mice infected with Plasmodium berghei ANKA/ PbA. Method: using 25 groups of BALB/C mice, divided into 5 groups: negative control group; positive control group and 4 treatment groups infected with PbA with extract dose of 150 mg / KgBW, 100 mg / KgBW, and 50 mg / KgBW for four days. On the fifth day the observation of liver tissue to determine deposition of hemozoin pigments by histopathological examination. Results: Statistical analysis showed that descriptively there was a decrease in hemozoin pigment in the liver tissue of mice (150 mg / KgBW extract dose decreased the most hemozoin pigment compared to other doses), but there was no statistically significant difference between the treatment groups with positive controls (p> 0.05). Conclusions: The administration of curcuma extract (Curcuma xanthorriza Roxb) has the potential to reduce the deposition of hemozoin pigment in liver tissue in BALB/C mice infected with Plasmodium berghei ANKA although statistically it is not significant.

Article Details

How to Cite
Utami, P., Dikwan, I., & Wibowo, A. (2020). Effect of Curcuma Xanthorriza Roxb Extract on Hemozoin Pigment Deposition In The Liver Tissue Of BALB /C Mice Infected With Plasmodium berghei ANKA . Indonesian Journal of Medical Sciences and Public Health           &Nbsp; , 1(1), 28-33. Retrieved from http://ijmpjournal.org/index.php/ijmp/article/view/3

References

1. The Ministry of Health Republic of Indonesia. (2015). National Malaria Control Program Strategic Plan 2015-2019. Retrieved from http://www.oilsearchhealthfoundation.org/wp-content/uploads/2014/01/NDOH-PNG-Malaria-National-Control-Strategic-Plan-2009-2013.pdf
2. Soulard, V., Bosson-Vanga, H., Lorthiois, A., Roucher, C., Franetich, J. F., Zanghi, G.,Mazier, D. (2015). Plasmodium falciparum full life cycle and Plasmodium ovale liver stages in humanized mice. Nature Communications, 6. (supl 7690) https://doi.org/10.1038/ncomms8690
3. Deroost, K., Lays, N., Pham, T. T., Baci, D., Van Den Eynde, K., Komuta, M., … Van Den Steen, P. E. (2014). Hemozoin induces hepatic inflammation in mice and is differentially associated with liver pathology depending on the Plasmodium strain. PLoS ONE, 9(11), 1–23. https://doi.org/10.1371/journal.pone.0113519
4. Jaijyan, D. K., & Singh, H. (2014). Malaria Liver Stage Parasites Strategies for Immune Evasion and Host Modulation : Implication for Vaccine Design. Austin Journal of Vaccines & Immunotherapeutics, 1(1), 1–5.
5. WHO. (2012). Update on artemisinin resistance - April 2012. World Health Organization, (November 2011), 1–10. Retrieved from http://www.who.int/malaria/publications/atoz/updateartemsininresistanceapr2012/en/
6. Haddad, M., Sauvain, M., & Deharo, E. (2011). Curcuma as a parasiticidal agent: A review. Planta Medica, 77(6), 672–678. https://doi.org/10.1055/s-0030-1250549
7. Lwin, K. M., Mon, H. M., & Myint, K. H. (2017). Evaluation of the antimalarial activity of Curcuma longa Linn ., singly and in combination with Eupatorium odoratum Linn . Journal of Ayurvedic and Herbal Medicine, 3(1), 11–14.
8. I Ihwah, A., Deoranto, P., Wijana, S., & Dewi, I. A. (2018). Comparative study between Federer and Gomez method for number of replication in complete randomized design using simulation: Study of Areca Palm (Areca catechu) as organic waste for producing handicraft paper. IOP Conference Series: Earth and Environmental Science, 131(1). https://doi.org/10.1088/1755-
9. Widyawaruyanti, A., Khasanah, U., Tumewu, L., Ilmi, H., Hafid, A. F., & Tantular, I. S. (2015). Antimalarial activity and cytotoxicity study of ethanol extract and fraction from Alectryon serratus leaves. International Journal of Pharmacy and Pharmaceutical Sciences, 7(12), 250–253. https://doi.org/10.1002/adsc.201600461
10. Adetutu, A., Olorunnisola, O. S., Owoade, A. O., & Adegbola, P. (2016). Inhibition of In Vivo Growth of Plasmodium berghei by Launaea taraxacifolia and Amaranthus viridis in Mice. Malaria Research and Treatment, 2016, 1–9.
11. Somsak, V., Polwiang, N., & Chachiyo, S. (2016). In Vivo Antimalarial Activity of Annona muricata Leaf Extract in Mice Infected with Plasmodium berghei. Journal of Pathogens, 2016, 1–5. https://doi.org/10.1155/2016/3264070
12. Minggarwati, T. S. (2017). Uji Aktivitas Antikanker dan Identifikasi Senyawa Aktif Dari Fraksi Umbi Bawang Sabrang (Eleutherine palmifolia(L.) Merr.) Terhadap Sel Kanker serviks HeLa. Repository Universitas Islam Negeri Maulana Malik Ibrahim Malang. https://doi.org/10.1002/ejsp.2570
13. He, Y., Yue, Y., Zheng, X., Zhang, K., Chen, S., & Du, Z. (2015). Curcumin, inflammation, and chronic diseases: How are they linked? Molecules, 20(5), 9183–9213. https://doi.org/10.3390/molecules20059183
14. Rohlan, K., Choudhary, S., Kumar, V., & Shringi, N. (2018). Embedding Techniques in Tissue Histological Process. In S. Ganguly & K. Rohlan (Eds.), Latest Trends in Zoology and Entomology Sciences Volume 1 (1st ed., pp. 38–42). New Delhi: AkiNik Publications, Rohini, Delhi, India. https://doi.org/10.22271/ed.book02.a04
15. Viriyavejakul, P., Khachonsaksumet, V., & Punsawad, C. (2014). Liver changes in severe Plasmodium falciparum malaria : histopathology , apoptosis and nuclear factor kappa B expression Liver changes in severe Plasmodium falciparum malaria : histopathology , apoptosis and nuclear factor kappa B expression. Malaria Journal, 13(1), 1–9. https://doi.org/10.1186/1475-2875-13-106
16. Pisciotta, J. M., Scholl, P. F., Shuman, J. L., Shualev, V., & Sullivan, D. J. (2017). Quantitative characterization of hemozoin in Plasmodium berghei and vivax. International Journal for Parasitology: Drugs and Drug Resistance, 7(1), 110–119. 1. The Ministry of Health Republic of Indonesia. (2015). National Malaria Control Program Strategic Plan 2015-2019. Retrieved from http://www.oilsearchhealthfoundation.org/wp-content/uploads/2014/01/NDOH-PNG-Malaria-National-Control-Strategic-Plan-2009-2013.pdf
2. Soulard, V., Bosson-Vanga, H., Lorthiois, A., Roucher, C., Franetich, J. F., Zanghi, G.,Mazier, D. (2015). Plasmodium falciparum full life cycle and Plasmodium ovale liver stages in humanized mice. Nature Communications, 6. (supl 7690) https://doi.org/10.1038/ncomms8690
3. Deroost, K., Lays, N., Pham, T. T., Baci, D., Van Den Eynde, K., Komuta, M., … Van Den Steen, P. E. (2014). Hemozoin induces hepatic inflammation in mice and is differentially associated with liver pathology depending on the Plasmodium strain. PLoS ONE, 9(11), 1–23. https://doi.org/10.1371/journal.pone.0113519
4. Jaijyan, D. K., & Singh, H. (2014). Malaria Liver Stage Parasites Strategies for Immune Evasion and Host Modulation : Implication for Vaccine Design. Austin Journal of Vaccines & Immunotherapeutics, 1(1), 1–5.
5. WHO. (2012). Update on artemisinin resistance - April 2012. World Health Organization, (November 2011), 1–10. Retrieved from http://www.who.int/malaria/publications/atoz/updateartemsininresistanceapr2012/en/
6. Haddad, M., Sauvain, M., & Deharo, E. (2011). Curcuma as a parasiticidal agent: A review. Planta Medica, 77(6), 672–678. https://doi.org/10.1055/s-0030-1250549
7. Lwin, K. M., Mon, H. M., & Myint, K. H. (2017). Evaluation of the antimalarial activity of Curcuma longa Linn ., singly and in combination with Eupatorium odoratum Linn . Journal of Ayurvedic and Herbal Medicine, 3(1), 11–14.
8. I Ihwah, A., Deoranto, P., Wijana, S., & Dewi, I. A. (2018). Comparative study between Federer and Gomez method for number of replication in complete randomized design using simulation: Study of Areca Palm (Areca catechu) as organic waste for producing handicraft paper. IOP Conference Series: Earth and Environmental Science, 131(1). https://doi.org/10.1088/1755-
9. Widyawaruyanti, A., Khasanah, U., Tumewu, L., Ilmi, H., Hafid, A. F., & Tantular, I. S. (2015). Antimalarial activity and cytotoxicity study of ethanol extract and fraction from Alectryon serratus leaves. International Journal of Pharmacy and Pharmaceutical Sciences, 7(12), 250–253. https://doi.org/10.1002/adsc.201600461
10. Adetutu, A., Olorunnisola, O. S., Owoade, A. O., & Adegbola, P. (2016). Inhibition of In Vivo Growth of Plasmodium berghei by Launaea taraxacifolia and Amaranthus viridis in Mice. Malaria Research and Treatment, 2016, 1–9.
11. Somsak, V., Polwiang, N., & Chachiyo, S. (2016). In Vivo Antimalarial Activity of Annona muricata Leaf Extract in Mice Infected with Plasmodium berghei. Journal of Pathogens, 2016, 1–5. https://doi.org/10.1155/2016/3264070
12. Minggarwati, T. S. (2017). Uji Aktivitas Antikanker dan Identifikasi Senyawa Aktif Dari Fraksi Umbi Bawang Sabrang (Eleutherine palmifolia(L.) Merr.) Terhadap Sel Kanker serviks HeLa. Repository Universitas Islam Negeri Maulana Malik Ibrahim Malang. https://doi.org/10.1002/ejsp.2570
13. He, Y., Yue, Y., Zheng, X., Zhang, K., Chen, S., & Du, Z. (2015). Curcumin, inflammation, and chronic diseases: How are they linked? Molecules, 20(5), 9183–9213. https://doi.org/10.3390/molecules20059183
14. Rohlan, K., Choudhary, S., Kumar, V., & Shringi, N. (2018). Embedding Techniques in Tissue Histological Process. In S. Ganguly & K. Rohlan (Eds.), Latest Trends in Zoology and Entomology Sciences Volume 1 (1st ed., pp. 38–42). New Delhi: AkiNik Publications, Rohini, Delhi, India. https://doi.org/10.22271/ed.book02.a04
15. Viriyavejakul, P., Khachonsaksumet, V., & Punsawad, C. (2014). Liver changes in severe Plasmodium falciparum malaria : histopathology , apoptosis and nuclear factor kappa B expression Liver changes in severe Plasmodium falciparum malaria : histopathology , apoptosis and nuclear factor kappa B expression. Malaria Journal, 13(1), 1–9. https://doi.org/10.1186/1475-2875-13-106
16. Pisciotta, J. M., Scholl, P. F., Shuman, J. L., Shualev, V., & Sullivan, D. J. (2017). Quantitative characterization of hemozoin in Plasmodium berghei and vivax. International Journal for Parasitology: Drugs and Drug Resistance, 7(1), 110–119. https://doi.org/10.1016/j.ijpddr.2017.02.001
17. Abu Lafi, S., Akkawi, M., Abu Remeleh, Q., Qutob, M., & Lutgen, P. (2019). Curcumin, a natural isolate from Curcuma longa (turmeric) with high β-hematin inhibitory potential. Pharmacy & Pharmacology International Journal, 7(1), 22–26. https://doi.org/10.15406/ppij.2019.07.00228
18. Panche, A. N., Diwan, A. D., & Chandra, S. R. (2016). Flavonoids: An overview. Journal of Nutritional Science, 5. https://doi.org/10.1017/jns.2016.41
19. Hewlings, S. J. (2017). Curcumin : A Review of Its ’ Effects on Human Health. Foods, 6(92), 1–11. https://doi.org/10.3390/foods6100092